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Cell Signaling Technology Inc
ps6 cell signaling 62016 mouse  Ps6 Cell Signaling 62016 Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/rnascope+probe+m%2E+musculus+wnt2/10__1172_slash_jci172116-313-27-28?v=Cell+Signaling+Technology+Inc Average 93 stars, based on 1 article reviews
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Journal: Journal of Clinical Investigation
Article Title: Hyperactive mTORC1 in lung mesenchyme induces endothelial cell dysfunction and pulmonary vascular remodeling
doi: 10.1172/jci172116
Figure Lengend Snippet: Figure 1. Characterization of pulmonary ECs from patients with LAM. Representative images of H&E and immunofluorescent-stained LAM lung (n=3) compared to age- and sex- matched control human lung (n=3) showing (A) remodeled distal vasculatures detected with antibodies against von Willebrand factor (vWF, red) and smooth muscle -actin (SMA, green) and (B) increased intimal fibrosis and medial thickening by H&E. DAPI detects nuclei (blue). (C) Representative images of dual immunofluorescent staining of human LAM lung (n=3) demonstrating loss of TSC2 (tuberin, magenta) expression in LAM lesion and concurrent activation of pS6 (marker of mTOR activation, green) and DAPI (nuclei; blue). *LAM micronodules. (D) Representative images of positive immunoreactivity for TSC2 protein (tuberin, magenta) and vWF (green) in ECs lining of the pulmonary vasculatures in both human control (n=4) and LAM lungs (n=4). DAPI (nuclei;
Article Snippet: Antibodies, reagents, and mRNA probes Antibody Company Catalog # Isotype Conc. (1) α-smooth muscle actin Sigma-Aldrich A2547 mouse 1:250 (2) PDGFRα Cell Signaling 3174 rabbit 1:100 (3)
Techniques: Staining, Control, Expressing, Activation Assay, Marker
Journal: Journal of Clinical Investigation
Article Title: Hyperactive mTORC1 in lung mesenchyme induces endothelial cell dysfunction and pulmonary vascular remodeling
doi: 10.1172/jci172116
Figure Lengend Snippet: Figure 5. Differential gene expression in the CD31+ vascular ECs from Tbx4LME_CreTsc2KO mouse lung. (A) Representative images of immunofluorescent staining of distal lung demonstrate positivity of pS6, a marker of mTORC1 upregulation (green) in PDGFR (red) mesenchymal cells of 8-week-old Tbx4LME_CreTsc2KO (Tsc2KO) mouse lungs. (B) Immunoblot of Tsc2 and pS6 proteins expression in Tsc2WT (n=3 in duplicates) and Tsc2KO (n=3 in duplicates) in 12-week- old mouse lung fibroblasts. (C) Statistical analysis of densitometry of Tsc2 and pS6 levels normalized to β-actin in each lane with average levels in Tsc2WT wild-type taken as 1. (D) Immunoblot of Tsc2 expression in lung ECs from Tsc2WT (n=3) and Tsc2KO (n=3) 8-week-old mice. (E) Statistical analysis of Tsc2 densitometry normalized to β-actin in each lane with the average expression levels in Tsc2WT set as 1. (F) Heatmap of the top differentially expressed genes in the mouse lung ECs isolated from Tsc2WT (n=3) and Tsc2KO (n=3) mice. (G) qPCR of Wnt pathway activation Axin2, ligands and receptors in ECs
Article Snippet: Antibodies, reagents, and mRNA probes Antibody Company Catalog # Isotype Conc. (1) α-smooth muscle actin Sigma-Aldrich A2547 mouse 1:250 (2) PDGFRα Cell Signaling 3174 rabbit 1:100 (3)
Techniques: Gene Expression, Staining, Marker, Western Blot, Expressing, Isolation, Activation Assay
Journal: Journal of Clinical Investigation
Article Title: Hyperactive mTORC1 in lung mesenchyme induces endothelial cell dysfunction and pulmonary vascular remodeling
doi: 10.1172/jci172116
Figure Lengend Snippet: Figure 6. Transcriptomic heterogeneity of ECs in Tbx4LME_CreTsc2KO mouse lung. (A) Uniform manifold approximation and projection (UMAP) representation of Tsc2WT and Tsc2KO mouse lungs (n=2) scRNA-seq with cell populations labelled according to the corresponding cell type. (B) ECs, marked by Pecam1 and vWF expression, were reclustered into 6 subclusters. (C) Mesenchymal cells, marked by Pdgfrα, Pdgfrβ and Msln expression, were reclustered into 8 subclusters. (D) Distribution of each EC type within the endothelial subclusters. (E) Gene Ontology (GO) enrichment analysis of arterial EC cluster. (F) WNT signaling pathway network (chord diagram) with incoming signal to arterial ECs from 3 mesenchymal populations including Axin2 myofibrogenic progenitor (AMP), Wnt2-Pdgfrα cells, and mesothelial cells. (G) Violin plot demonstrating increased expression of Bmp4, Pparg, and Sox17 in arterial (AECs) from Tsc2KO mice compared to arterial ECs from Tsc2WT. (H) Representative images of 54-week-old Tsc2WT (n=3) and Tsc2KO (n=3) for pS6 (marker of mTORC1 upregulation; magenta); light green reflects an autofluorescence from structural proteins in lung mesenchyme. DAPI (blue) detects nuclei. (I) Representative images of dual staining of 54-week-old Tsc2WT (n=3) and Tsc2KO (n=3) lung for Axin2 mRNA (magenta) and pS6 (green). (J) Quantification of Axin2 mRNA shown in (I). (K) Pulmonary vasculature in both Tsc2WT and Tsc2KO with Wnt2 mRNA (magenta) and pS6 (green) from single-molecule fluorescent in situ hybridization). (L) Quantification of Wnt2 mRNA shown in (K). Statistical analysis was performed using two-tailed t tests and data are represented as mean ± SEM. * P<0.05; ** P<0.01; *** P<0.001; **** P<0.0001.
Article Snippet: Antibodies, reagents, and mRNA probes Antibody Company Catalog # Isotype Conc. (1) α-smooth muscle actin Sigma-Aldrich A2547 mouse 1:250 (2) PDGFRα Cell Signaling 3174 rabbit 1:100 (3)
Techniques: Expressing, Marker, Staining, In Situ Hybridization, Two Tailed Test